Plasmid
Part:BBa_K4706019:Design
Designed by: Niklas Küppers Group: iGEM23_Duesseldorf (2023-10-01)
pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependent cell death
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3049
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3049
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3055 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3049
Illegal BglII site found at 2500
Illegal BglII site found at 4476
Illegal BglII site found at 4824
Illegal BamHI site found at 3613
Illegal XhoI site found at 1533
Illegal XhoI site found at 2425 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3049
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3049
Illegal XbaI site found at 3064
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3597
Design Notes
pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependet cell death
Source
pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependet cell death